Ciloa technology

 

Ciloa's membrane proteins (GPCRs, ion channels, viral envelope proteins…) are naturally expressed in cells and sorted embedded in exosome membranes with their fully native conformation

Membrane proteins

The transmission of signals and of metabolites through the membrane of all organisms is absolutely required for their life and their survival. Proteins penetrating the lipid bilayer of membranes are responsible of these vital connections between internal and external environments. Due to this strategic location, integral membrane proteins  (GPCRs, ion channels, transporters…) are the main targets for both actual and future drugs used in prevention and therapies.

Ciloa's focus on membrane proteins on exosomes

For the development of new therapies or preventive strategies, it would be useful to have membrane proteins (GPCRs, ion channels, viral envelope proteins…) in the purest form but still in native conformation and embedded in a membrane. In contrast to soluble proteins that are quite easy to produce, this is very challenging for integral membrane proteins; indeed, they lay on different mutually incompatible environments, i.e. "aqueous-lipidic-aqueous". The influence of membrane phospholipids on membrane proteins is especially significant. In absence of such specific environments, membrane proteins do not fold properly and consequently are not functional. In addition, most membrane proteins must acquire specific post-translational modifications for their proper folding and several are active as homo- or hetero-oligomers. Membrane proteins on exosomes are embedded in a natural  membrane originated from cells and present a topology perfectly identical to the membrane proteins exposed at the cell surface.



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