Fully native membrane antigens (GPCRs, receptor kinases, adhesion molecules, ion channels, viral envelope proteins ...) on natural nanovesicles called exosomes
are known to be potent immuno-stimulators
. These exosomes allow to develop antibodies via both immunization (in vivo
originating antibodies) and selection of phage libraries (in vitro
originating antibodies). In addition, screening and characterization of monoclonal antibodies
are easy using ELISA and SPR on membrane proteins presented by exosomes.
No pathogens are necessary to develop vaccines against tumor cells, viruses, bacteria or parasites using membrane proteins (GPCRs, viral envelope proteins …) sorted in their native conformation on exosomes
. Exosomes are potent immuno-stimulators
able to trigger protective immune response. The combination of both, a perfect antigen and a potent natural adjuvant, leads to the perfect candidate vaccine. Ciloa develops a proprietary pipeline of preventive & therapeutic vaccines
based on exosomes.
Native transmembrane proteins on exosomes
make possible to develop new kinds of diagnostic
tests to detect molecules that are specific to the conformational structure of membrane proteins. Several tags, such as biotin, GFP, hexa-His, and more, can be added on the targeted membrane proteins to help detection. Detection can be performed using classic ELISA techniques or SPR.